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little DOMS — Database Findings

Note: This is a temporary planning document that will be deleted when the investigation is complete.

Date: 2026-03-07 Investigator accounts: - Althea: althea.tomas@gmail.com (F5XWaUIex06j3KycXnpSUA==) - Maria: maria.arroyo@charite.de (xr22lv0TQESE9BNC69axeA==)


Project: little DOMS

Created: 2021-02-02

Stages

# Name Extraction Questions
1 Data extraction true 128
2 Risk of bias assessment false 18 (17 shared with Stage 1)

Althea's 6 Affected Studies — Sessions + Annotations

All annotations currently have Stage 1 stageId provenance.

Study (abbreviated) S1 Session Created S2 Session Created Annotations Written Ann Count
Low-dose nifedipine 2026-02-03 16:55 completed 2026-02-18 19:20 completed 2026-02-25 17:51 52 root + 134 non-root = 186
NLRP3 Inflammasome 2026-02-03 16:50 completed 2026-02-18 19:11 completed 2026-02-25 17:31 47 root + 97 non-root = 144
Contralesional angiotensin 2026-02-05 12:04 completed 2026-02-18 18:17 completed 2026-02-25 16:11 55 root + 213 non-root = 268
Huang-Lian-Jie-Du 2026-02-05 15:57 completed 2026-02-19 12:01 completed 2026-03-05 10:29 48 root + 97 non-root = 145
New alternative approaches 2026-02-09 12:46 completed 2026-02-18 19:01 completed 2026-03-05 10:42 56 root + 225 non-root = 281
DPP-4 Linagliptin 2026-02-09 15:53 completed 2026-02-18 19:31 completed 2026-03-05 10:59 52 root + 193 non-root = 245

Critical: every study has a single annotation timestamp — all annotations were written in one atomic submission per study.


Maria's 7 Studies — Sessions + Annotations

Maria has no Stage 2 sessions anywhere.

Study (abbreviated) S1 Session Created Annotations Written Ann Count
ESO-WSO 2020 2026-02-18 14:38 completed 2026-02-18 14:38 31+1 = 32 (excluded study)
Carvedilol 2026-02-19 15:32 in-progress 2026-02-19 15:32 15+1 = 16 (partial)
Huang-Lian-Jie-Du 2026-02-18 15:19 completed 2026-02-24 12:19 46+88 = 134
FGF21 2026-02-24 12:35 completed 2026-02-24 12:36 39+1 = 40 (excluded study)
Lauric acid 2026-02-24 12:50 completed 2026-02-24 12:50 39+1 = 40 (excluded study)
Contralesional angiotensin 2026-02-24 14:08 completed 2026-02-25 14:55 45+88 = 133
New alternative approaches 2026-02-26 13:15 completed 2026-02-26 14:38 56+216 = 272

Annotation Question Tree Structure

The tree, derived from the annotation root/non-root relationships:

ROOT (study-level)
├── [Metadata] English study, Is the study excluded?*, Conflict of interest,
│   Has a protocol been published?, How many animals were used...
├── [Stage 1 procedure labels — each has many children]
│   ├── Cohort label?
│   │   └── [children] Species, Strain, Animal Sex, Weight [g] (upper/lower),
│   │       Age of animals, Source of animals, Type/Was comorbidity confirmed,
│   │       How is diabetes mellitus induced?, Reported excluded animals...
│   │
│   ├── Experiment label?
│   │   └── [children] Disease Models, Cohorts, Treatments (with further nesting)
│   │       └── [within Treatments] Treatment name, Dose, Route, Intervention type,
│   │                               Number of interventions, Co-treatment...
│   │
│   ├── Outcome assessment procedure label?
│   │   └── [children] What is the name of the neurobehavioral outcome?,
│   │       Outcome measure, Outcome measure units, Type, average/error type,
│   │       Greater is worse, Reference group, Function addressed...
│   │       ├── Item 6 – Detection bias: Random outcome assessment  <- per outcome
│   │       ├── Item 7 – Detection bias: Blinding                  <- per outcome
│   │       └── Item 8 – Attrition bias: Incomplete outcome data    <- per outcome
│   │
│   └── Disease model induction procedure label?
│       └── [children] Model used to induce diabetes?, Type of diabetes modelled?,
│           Was timing of disease induction random?...
├── [Stage 1 reporting questions]
│   Reporting of excluded animals?, Were investigators blinded?,
│   Were the animals randomly housed?, Animal housing pre/post-stroke?,
│   Prespecification of in-/exclusion criteria?, etc.
└── [RoB items — study-level, shared with Stage 2]
    ├── Item 1 – Selection bias: Sequence generation
    ├── Item 2 – Selection bias: Baseline characteristics (two variants)
    ├── Item 3 – Selection bias: Allocation concealment
    ├── Item 4 – Performance bias: Random housing
    ├── Item 5 – Performance bias: Blinding
    ├── Item 9 – Reporting bias: Selective outcome reporting
    ├── Item 10 - Other: Other sources of bias
    └── Is this study reconciled

* "Is the study excluded?" root annotation has 1 non-root child (the exclusion reason).


Chronology — What Actually Happened

2021-02-02  Project "little DOMS" created

2026-02-03  Althea starts Stage 1 (Data Extraction) on NLRP3 and Low-dose nifedipine
2026-02-05  Althea starts Stage 1 on Contralesional angiotensin and Huang-Lian-Jie-Du
2026-02-09  Althea starts Stage 1 on New alternative approaches and DPP-4 Linagliptin
            -> At this point Althea has ~268-281 annotations per study saved under Stage 1

2026-02-12  * Althea files #2335 — wide format export crashes with ArgumentNullException
            (null StringArrayAnnotation — separate bug, latent since ~2021)

2026-02-18  Althea completes Stage 2 (RoB) for 5 studies within the same evening:
              18:17 — Contralesional angiotensin Stage 2 session created (completed)
              19:01 — New alternative approaches Stage 2 session created (completed)
              19:11 — NLRP3 Stage 2 session created (completed)
              19:20 — Low-dose nifedipine Stage 2 session created (completed)
              19:31 — DPP-4 Linagliptin Stage 2 session created (completed)
            -> Stage 2 submission tree-shakes Stage 1's 17 shared-question annotations
               and replaces them with Stage 2 RoB answers

            Maria starts Stage 1:
              14:38 — ESO-WSO 2020 (completed, excluded study, 32 annotations)
              15:19 — Huang-Lian-Jie-Du session created (annotations written later)

2026-02-19  Althea completes Stage 2 for Huang-Lian-Jie-Du (12:01)
            * Althea files #2337 — "Outcome assessment level inputs have disappeared"
              -> She completed Stage 2 yesterday/today and the outcome-level annotations
                 are now missing when she views Stage 2

            Maria starts Stage 1 on Carvedilol (15:32) — still in-progress today

2026-02-20  * Maria files #2339 — "same export crash + completing RoB corrupts outcome
              annotations in Data Extraction"
              -> Maria has no Stage 2 sessions: she is either reporting what she observed
                 happening to the project, or tried Stage 2 but it didn't save a session

2026-02-24  Maria annotates three more Stage 1 studies:
              12:19 — Huang-Lian-Jie-Du annotations written (session from 18 Feb)
              12:35 — FGF21 session created + 40 annotations (excluded)
              12:50 — Lauric acid session + 40 annotations (excluded)
              14:08 — Contralesional angiotensin session created

2026-02-25  Maria writes annotations for Contralesional angiotensin at 14:55 (133 total)

            ** Althea re-saves Stage 1 for 3 studies — ALL annotations completely
               rewritten in single submissions (Stage 1 form, all 128 questions):
              16:11 — Contralesional angiotensin: 268 annotations rewritten
              17:31 — NLRP3: 144 annotations rewritten
              17:51 — Low-dose nifedipine: 186 annotations rewritten

2026-02-26  Maria writes New alternative approaches annotations (272 total)

2026-03-05  ** Althea re-saves Stage 1 for remaining 3 studies:
              10:29 — Huang-Lian-Jie-Du: 145 annotations rewritten
              10:42 — New alternative approaches: 281 annotations rewritten
              10:59 — DPP-4 Linagliptin: 245 annotations rewritten

What This All Means

The most significant finding is that every one of Althea's 6 affected studies has a single annotation timestamp — meaning ALL annotations (not just the 17-18 shared questions) were completely overwritten in a single Stage 1 submission per study, roughly 6–14 days after Stage 2 was completed.

This points to Althea manually going back into Stage 1 and re-submitting everything — not an auto-save. She did 3 studies in one session on 25 Feb (over ~1.5 hours), then the other 3 on 5 Mar (over ~30 minutes). She was clearly working through the list deliberately.

What happened to the original Stage 2 annotations? Between Stage 2 completion (18-19 Feb) and Althea's re-annotation (25 Feb / 5 Mar), the outcome-level annotations were in an unknown state — this is exactly what Althea reported as missing on 19 Feb. The 25 Feb / 5 Mar data is Althea's second attempt, not the original Stage 2 work.

The question you cannot answer from the current database: whether Althea's re-entered RoB values match her original Stage 2 work. The Atlas backup from 21 Feb would show what state the annotations were in immediately after Stage 2 completed (and potentially after they disappeared) — and comparing that to the current data would tell you whether anything was actually lost or altered.

Maria's data is unaffected — she only ever did Stage 1, her 7 studies all have consistent single-timestamp annotations, and her data looks structurally complete for the studies she finished.